Journal:

Article Title: Lack of metallothionein-I and -II exacerbates the immunosuppressive effect of ultraviolet B radiation and cis -urocanic acid in mice

doi: 10.1046/j.1365-2567.2000.00026.x

Figure Lengend Snippet: Immunostaining for MT in the skin of MT+/+ mice (a and b) and MT–/– mice (c and d). Untreated skin (a and c, ×50 magnification) shows weak staining in MT+/+ epidermis, non-specific background staining in MT–/– epidermis. At 72 hr post-UVB irradiation (b and d, ×100 magnification) epidermal hyperplasia is evident in both mouse strains; strong MT expression has been induced in MT+/+ epidermis (b), but there is only background staining for MT in MT–/– epidermis (d).

Article Snippet: 19 The bound primary antibody was then visualized with the avidin–biotin peroxidase complex (ABC) immunostaining method (PK-4000,Vector Lab., Burlingame, CA) as previously described.

Techniques: Immunostaining, Staining, Irradiation, Expressing

Journal: Neoplasia (New York, N.Y.)

Article Title: p53-induced apoptosis occurs in the absence of p14(ARF) in malignant pleural mesothelioma.

doi: 10.1593/neo.06148

Figure Lengend Snippet: Figure 1. Expression levels of p14ARF in MPM cell lines. RNA was isolated from MPM primary cultures and cell lines and SV40-transformed Met5A cells, and RT-PCR was performed to detect p14ARF, SV40 L-Tag and GAPDH mRNA. Lysates of the same cell lines were analyzed by immunoblotting using anti-p14ARF C-terminal antibodies. Staining of actin was used as equal loading control.

Article Snippet: The Met5A cell line (Dr. J. F. Lechner; NCI, Bethesda, MD) corresponds to normal human mesothelial cells immortalized by transfection with an SV40 early region plasmid [19], and the NCI-H28 cell line was obtained from ATCC (Manassas, VA).

Techniques: Expressing, Isolation, Transformation Assay, Reverse Transcription Polymerase Chain Reaction, Western Blot, Staining, Control

Journal: Developmental biology

Article Title: MicroRNA-200c-141 and ∆Np63 are required for breast epithelial differentiation and branching morphogenesis.

doi: 10.1016/j.ydbio.2015.05.007

Figure Lengend Snippet: Fig. 3. Overexpression of miR-200c-141 in D492 inhibits EMT, but does not affect branching. (A) qPCR analysis verifies the overexpression of miR-200c and -141 in D492. D492M cells were transduced with a lentiviral construct containing miR-200c-141 (D492miR-200c-141). Expression levels of miR-200c and -141 were 2–3 fold higher in D492miR-200c-141 than in D492empty cells. miRNA levels were normalized to U6. (B) D492miR-200c-141 cells cultured in rBM monoculture form branching colonies similar to D492empty. (C) D492miR-200c-141 are resistent to EMT in coculture with endothelial cells. D492miR-200c-141 only form branching structures in coculture with BRENCS while D492empty form branching and mesenchymal colonies in coculture.

Article Snippet: ΔNp63 expression was downregulated using shp63alpha pLKO.1 puro lentiviral vector (Addgene plasmid 19,120 (Godar et al., 2008)) and pLKO.1 shSCR lentiviral vector (Addgene plasmid 17,920 (Saharia et al., 2008)) was used as control.

Techniques: Over Expression, Transduction, Construct, Expressing, Cell Culture

Journal: Developmental biology

Article Title: MicroRNA-200c-141 and ∆Np63 are required for breast epithelial differentiation and branching morphogenesis.

doi: 10.1016/j.ydbio.2015.05.007

Figure Lengend Snippet: Fig. 4. Forced expression of miR-200c-141 induces luminal epithelial differentiation. (A) Overexpression of miR-200c-141 in D492M induces epithelial morphology in 2D culture. D492M cells were transduced with a lentiviral construct containing miR-200c-141 (D492MmiR-200c-141). The expression was 300–500 fold higher in D492MmiR-200c-141

Article Snippet: ΔNp63 expression was downregulated using shp63alpha pLKO.1 puro lentiviral vector (Addgene plasmid 19,120 (Godar et al., 2008)) and pLKO.1 shSCR lentiviral vector (Addgene plasmid 17,920 (Saharia et al., 2008)) was used as control.

Techniques: Expressing, Over Expression, Transduction, Construct

Journal: Developmental biology

Article Title: MicroRNA-200c-141 and ∆Np63 are required for breast epithelial differentiation and branching morphogenesis.

doi: 10.1016/j.ydbio.2015.05.007

Figure Lengend Snippet: Fig. 5. Forced expression of ΔNp63 induces myoepithelial differentiation. (A) ΔNp63 overexpression has little effect on morphology of D492M in 2D culture. D492M cells were transduced with a lentiviral construct containing ΔNp63 (D492MΔNp63). The expression levels of ΔNp63 were 50 fold higher in D492MΔNp63 than in D4922 empty cells. Phase contrast image of D492MΔNp63 shows minor effect of ΔNp63 overexpression on cell morphology. miRNA levels were normalized to U6. Bar¼100 mM. (B) D492MΔNp63

Article Snippet: ΔNp63 expression was downregulated using shp63alpha pLKO.1 puro lentiviral vector (Addgene plasmid 19,120 (Godar et al., 2008)) and pLKO.1 shSCR lentiviral vector (Addgene plasmid 17,920 (Saharia et al., 2008)) was used as control.

Techniques: Expressing, Over Expression, Transduction, Construct

Journal: Developmental biology

Article Title: MicroRNA-200c-141 and ∆Np63 are required for breast epithelial differentiation and branching morphogenesis.

doi: 10.1016/j.ydbio.2015.05.007

Figure Lengend Snippet: Fig. 6. Co-expression of mir-200c-141 and ΔNp63 induces LEP and MEP differentiation. (A) Co-expression of miR-200c-141 and ΔNp63 in D492M. D492MmiR-200c-141 were transduced with a lentiviral construct containing ΔNp63 (D492MmiR-200c-141-ΔNp63). Phase contrast image of D492MmiR-200c-141-ΔNp63 shows cuboidal epithelial phenotype in culture. miR-200c, miR-141 and ΔNp63 expression levels were 50–1700 fold higher in D492MmiR-200c-141-ΔNp63 than in D4922 empty cells, respectively. miRNA levels were normalized to U6. Bar¼100 mM. (B) miR-200c-141 and ΔNp63 coexpression induces expression of luminal and myoepithelial markers. Western blot shows strong expression of the myoepithelial markers p63, K5/6, K17 and P-cadherin and a vague K14 expression in D492MmiR-200c-141-ΔNp63. The luminal markers EpCAM and K19 are also expressed. Actin as loading control. (C) D492MmiR-200c-141-ΔNp63 cells in rBM monoculture form irregular branching colonies. Immunostaining shows co-expression of N-cad (green) and E-cad (red) D492MmiR-200c-141-Δp63. Note the lack of polarity in N-cad expression (compared with Fig. 1A). D492MmiR-200c-141-ΔNp63 expresses both luminal marker K19 (red) and myoepithelial marker K17 (green). Cells were counterstained with TO-PRO-3 nuclearstain. Bar¼100 mM.

Article Snippet: ΔNp63 expression was downregulated using shp63alpha pLKO.1 puro lentiviral vector (Addgene plasmid 19,120 (Godar et al., 2008)) and pLKO.1 shSCR lentiviral vector (Addgene plasmid 17,920 (Saharia et al., 2008)) was used as control.

Techniques: Expressing, Transduction, Construct, Western Blot, Control, Immunostaining, Marker